B-mode ultrasound characterization of myofascial trigger points and their response to physiotherapy interventions: a systematic review
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Abstract
Background and objectives: Myofascial pain syndrome, provoked by myofascial trigger points (MTrPs), is a common cause of musculoskeletal pain. Accurate identification of MTrPs remains difficult due to its subjective clinical assessment. B-mode ultrasound (US) offers a promising alternative for characterizing MTrPs, although its clinical utility remains uncertain. This study aimed to evaluate the evidence supporting B-mode US to characterize MTrPs, assess changes in muscle architecture, and analyze the impact of physiotherapeutic interventions.
Methods: A systematic review was registered in PROSPERO following PRISMA guidelines (CRD42024596408). PubMed, Scopus and Web of Science databases were searched in October 2024. Variables of interest included MTrP characteristics (hypo/hyperechoic areas, dimensions, and morphology), muscle architecture (e.g., muscle thickness or pennation angle), and ecotexture analysis (first-, second-, and higher-order features).
Results: From 28 eligible studies and 929 participants, MTrPs were consistently identified as predominantly hypoechoic nodules with heterogeneous echotexture and elliptical shapes, with areas from 0.02 to 3.4 cm². Variations in muscle architecture, such as reduced thickness, and echotexture, mainly characterized by reduced echogenicity, were frequent in affected muscles by MTrPs. Physiotherapy interventions showed variable efficacy in altering MTrP characteristics depending on the intervention type and population. Methodological quality ranged from poor/low to good/high, with a moderate risk of bias.
Discussion: This review highlights the potential of B-mode US as an assessment tool for MTrPs. However, inconsistencies in imaging protocols, limited quantitative analyses, and the intrinsic characteristics of the included study designs, make it difficult to draw definitive conclusions.
Conclusions: Although B-mode US shows promise in characterizing MTrPs, methodological limitations highlight the need for further studies of higher methodological quality. Standarizing US assessment protocols for MTrPs could improve diagnostic accuracy and treatment monitoring and benefit clinical practice.
Keywords
- Myofascial Trigger Point
- Systematic Review
- musculoskeletal pain
- ultrasonography
- physical therapy
References
Langley PC, Molina JT, Ferri CM, Pérez Hernández C, Varillas AT, Angel Ruiz-Iban M. The association of pain with labor force participation, absenteeism, and presenteeism in Spain. J Med Econ. 2011;14. https://doi.org/10.3111/13696998.2011.632045
Fleckenstein J, Zaps D, Rüger LJ, Lehmeyer L, Freiberg F, Lang PM, Irnich D. Discrepancy between prevalence and perceived effectiveness of treatment methods in myofascial pain syndrome: Results of a cross-sectional, nationwide survey. BMC Musculoskelet Disord. 2010;11. https://doi.org/10.1186/1471-2474-11-32
Gerwin RD. Diagnosis of myofascial pain syndrome. Phys Med Rehabil Clin N Am. 2014;25. https://doi.org/10.1016/j.pmr.2014.01.011
Shah JP, Thaker N, Heimur J, Aredo J V., Sikdar S, Gerber L. Myofascial trigger points then and now: A historical and scientific perspective. PM R. 2015;7. https://doi.org/10.1016/j.pmrj.2015.01.024
Simons DG. Diagnostic criteria of myofascial pain caused by trigger points. J Musculoskelet Pain. 1999;7. http://dx.doi.org/10.1300/J094v07n01_11 DOI: https://doi.org/10.1300/J094v07n03_08
Fundación Grünenthal, Observatorio del dolor de la Universidad de Cádiz, BioInnova Consulting. Barómetro del dolor crónico en España 2022. Available from: https://www.fundaciongrunenthal.es/fundacion/con-la-ciencia/barometro-dolor-cronico-espana-2022
Gregory NS, Sluka KA. Anatomical and physiological factors contributing to chronic muscle pain. Curr Top Behav Neurosci. 2014;20. https://doi.org/10.1007/7854_2014_294
Barbero M, Schneebeli A, Koetsier E, Maino P. Myofascial pain syndrome and trigger points: Evaluation and treatment in patients with musculoskeletal pain. Curr Opin Support Palliat Care. 2019;13. https://doi.org/10.1097/spc.0000000000000445
Lucas N, Macaskill P, Irwig L, Moran R, Bogduk N. Reliability of Physical Examination for Diagnosis of Myofascial Trigger Points: A Systematic Review of the Literature. Clin J Pain. 2008;25. https://doi.org/10.1097/ajp.0b013e31817e13b6
Myburgh C, Larsen AH, Hartvigsen J. A Systematic, Critical Review of Manual Palpation for Identifying Myofascial Trigger Points: Evidence and Clinical Significance. Arch Phys Med Rehabil. 2008;89. https://doi.org/10.1016/j.apmr.2007.12.033
Simons DG. New Views of Myofascial Trigger Points: Etiology and Diagnosis. Arch Phys Med Rehabil. 2008;89. https://doi.org/10.1016/j.apmr.2007.11.016
Elbarbary M, Sgro A, Goldberg M, Tenenbaum H, Azarpazhooh A. Diagnostic Applications of Ultrasonography in Myofascial Trigger Points: A Scoping Review and Critical Appraisal of Literature. J Diagn Med Sonogr. 2022;38. https://doi.org/10.1177/87564793221102593
Mazza DF, Boutin RD, Chaudhari AJ. Assessment of Myofascial Trigger Points via Imaging: A Systematic Review. Am J Phys Med Rehabil. 2021;100. https://doi.org/10.1097/phm.0000000000001789
Ricci V, Mezian K, Chang KV, Tarantino D, Güvener O, Gervasoni F, Naňka O, Özçakar L. Ultrasound Imaging and Guidance for Cervical Myofascial Pain: A Narrative Review. Int J Environ Res Public Health. 2023;20. https://doi.org/10.3390/ijerph20053838
Basford JR, An KN. New techniques for the quantification of fibromyalgia and myofascial pain. Curr Pain Headache Rep. 2009;13. https://doi.org/10.1007/s11916-009-0061-6
Sikdar S, Shah JP, Gebreab T, Yen RH, Gilliams E, Danoff J, Gerber LH. Novel Applications of Ultrasound Technology to Visualize and Characterize Myofascial Trigger Points and Surrounding Soft Tissue. Arch Phys Med Rehabil. 2010;90. https://doi.org/10.1016/j.apmr.2009.04.015
Ricci V, Ricci C, Gervasoni F, Cocco G, Andreoli A, Özçakar L. From Histoanatomy to Sonography in Myofascial Pain Syndrome: A EURO-MUSCULUS/USPRM Approach. Am J Phys Med Rehabil. 2023;102. https://doi.org/10.1097/phm.0000000000001975
Srbely JZ, Kumbhare D, Grosman-Rimon L. A narrative review of new trends in the diagnosis of myofascial trigger points: Diagnostic ultrasound imaging and biomarkers. J Can Chiropr Assoc. 2016;60. https://pmc.ncbi.nlm.nih.gov/articles/PMC5039775/
Thomas K, Shankar H. Targeting myofascial taut bands by ultrasound topical collection on myofascial pain. Curr Pain Headache Rep. 2013;17. https://doi.org/10.1007/s11916-013-0349-4
Kumbhare DA, Elzibak AH, Noseworthy MD. Assessment of Myofascial Trigger Points Using Ultrasound. Am J Phys Med Rehabil. 2016;95. https://doi.org/10.1097/phm.0000000000000376
Page MJ, McKenzie JE, Bossuyt PM, Boutron I, Hoffmann TC, Mulrow CD, Shamseer L, Tetzlaff JM, Akl EA, Brennan SE, Chou R, Glanville J, Grinshaw JM, Hróbjartsson A, Lalu, MM, Li T, Loder EW, Mayo-Wilson E, McDonald S, McGuinness LA, Stewart LA, Thomas J, Tricco AC, Welch VA, Whiting P, Moher D. The PRISMA 2020 statement: an updated guideline for reporting systematic reviews. BMJ. 2021;372. https://doi.org/10.1136/bmj.n71
Albanese E, Bütikofer L, Armijo-Olivo S, Ha C, Egger M. Construct validity of the Physiotherapy Evidence Database (PEDro) quality scale for randomized trials: Item response theory and factor analyses. Res Synth Methods. 2020;11. https://doi.org/10.1002/jrsm.1385
Sterne JA, Hernán MA, Reeves BC, Savović J, Berkman ND, Viswanathan MM, Henry D, Altman DG, Ansari MT, Boutron I, Carpenter JR, Chan AW, Churchill R, Deeks JJ, Hróbjartsson A, Kirkham J, Jüni P, Loke YK, Pigott TD, Ramsay CR, … Higgins JP. ROBINS-I: a tool for assessing risk of bias in non-randomised studies of interventions. BMJ. 2016;355. https://doi.org/10.1136/bmj.i4919
Study Quality Assessment Tools | NHLBI, NIH. Available from: https://www.nhlbi.nih.gov/health-topics/study-quality-assessment-tools
Owen H. Case study: Treating infraspinatus and supraspinatus trigger points and supraspinatus tendinopathy utilizing piezoelectric shockwave. Front Vet Sci. 2022;9. https://doi.org/10.3389/fvets.2022.943276
Ball A, Perreault T, Fernández-De-las-peñas C, Agnone M, Spennato J. Ultrasound Confirmation of the Multiple Loci Hypothesis of the Myofascial Trigger Point and the Diagnostic Importance of Specificity in the Elicitation of the Local Twitch Response. Diagnostics (Basel). 2022;12. https://doi.org/10.3390/diagnostics12020321
Cankurtaran D, Aykın Yığman Z, Güzel Ş, Umay E. The importance of myofascial trigger points in chronic neck pain: An ultrasonography preliminary study. PM R. 2023;15. https://doi.org/10.1002/pmrj.12974
Da Silva AC, Aily JB, Oliveira AB, Mattiello SM. Interrater and Intrarater Reliability and Minimum Detectable Change of Ultrasound for Active Myofascial Trigger Points in Upper Trapezius Muscle in Individuals With Shoulder Pain. J Manipulative Physiol Ther. 2020;43. https://doi.org/10.1016/j.jmpt.2020.01.003
Elbarbary M, Goldberg M, Tenenbaum HC, Lam DK, Freeman B V., Pustaka DJ, Mock D, Beyene J, Azarpazhooh A. Assessment of Concordance between Chairside Ultrasonography and Digital Palpation in Detecting Myofascial Trigger Points in Masticatory Myofascial Pain Syndrome. J Endod. 2023;49. https://doi.org/10.1016/j.joen.2022.11.013
Kumbhare D, Shaw S, Grosman-Rimon L, Noseworthy MD. Quantitative Ultrasound Assessment of Myofascial Pain Syndrome Affecting the Trapezius: A Reliability Study. J Ultrasound Med. 2017;36. https://doi.org/10.1002/jum.14308
Kumbhare D, Shaw S, Ahmed S, Noseworthy MD. Quantitative ultrasound of trapezius muscle involvement in myofascial pain: comparison of clinical and healthy population using texture analysis. J Ultrasound. 2020;23. https://doi.org/10.1007/s40477-018-0330-5
Shankar H, Reddy S. Two- and three-dimensional ultrasound imaging to facilitate detection and targeting of taut bands in myofascial pain syndrome. Pain Med. 2012;13. https://doi.org/10.1111/j.1526-4637.2012.01411.x
Taheri N, Okhovatian F, Rezasoltani A, Karami M, Hosseini SM, Mohammadi HK. Ultrasonography in diagnosis of myofascial pain syndrome and reliability of novel ultrasonic indexes of upper trapezius muscle. Ortop Traumatol Rehabil. 2016;18. https://doi.org/10.5604/15093492.1205022
Takla MKN, Razek NMA, Kattabei O, El-Lythy MAF. A comparison between different modes of real-time sonoelastography in visualizing myofascial trigger points in low back muscles. J Man Manip Ther. 2016;24. https://doi.org/10.1179/2042618614y.0000000084
Turo D, Otto P, Shah JP, Heimur J, Gebreab T, Zaazhoa M, Armstrong K, Gerber LH, Sikdar S. Ultrasonic characterization of the upper trapezius muscle in patients with chronic neck pain. Ultrason Imaging. 2013;35. https://doi.org/10.1177/0161734612472408
Zale KE, Klatt M, Volz KR, Kanner C, Evans KD. A Mixed-Method Approach to Evaluating the Association between Myofascial Trigger Points and Ankle/Foot Pain Using Handheld Sonography Equipment. J Diagn Med Sonogr. 2015;31. https://doi.org/10.1177/8756479315586396
Adigozali H, Shadmehr A, Ebrahimi E, Rezasoltani A, Naderi F. B mode, doppler and ultrasound elastography imaging on active trigger point in women with myofascial pain syndrome treated by dry needling. Muscles Ligaments Tendons J. 2019;9. http://dx.doi.org/10.32098/mltj.03.2019.16
Ceylan CM, Korkmaz MD, Corum M, Kesiktas FN. Demonstration of kinesio taping effect by ultrasonography in neck pain. Rev Assoc Med Bras (1992). 2022;68. https://doi.org/10.1590/1806-9282.20220668
Korkmaz MD, Medin Ceylan C. Effect of dry-needling and exercise treatment on myofascial trigger point: A single-blind randomized controlled trial. Complement Ther Clin Pract. 2022;47. https://doi.org/10.1016/j.ctcp.2022.101571
Conte da Silva A, Aily JB, Mattiello SM. Ischemic compression associated with joint mobilization does not promote additional clinical effects in individuals with rotator cuff related shoulder pain: A randomized clinical trial. J Bodyw Mov Ther. 2023;36. https://doi.org/10.1016/j.jbmt.2023.08.002
Hadizadeh M, Rahimi A, Javaherian M, Velayati M, Naderi F, Dommerholt J. The Effects of Intramuscular Electrical Stimulation on Clinical and Sonographic Parameters in the People with Trigger Points: A Case Series Study. J Rehabil. 2023;24. http://dx.doi.org/10.32598/RJ.24.3.3653.1
Jafari M, Bahrpeyma F, Togha M. Effect of ischemic compression for cervicogenic headache and elastic behavior of active trigger point in the sternocleidomastoid muscle using ultrasound imaging. J Bodyw Mov Ther. 2017;21. https://doi.org/10.1016/j.jbmt.2017.01.001
Togha M, Bahrpeyma F, Jafari M, Nasiri A. A sonographic comparison of the effect of dry needling and ischemic compression on the active trigger point of the sternocleidomastoid muscle associated with cervicogenic headache: A randomized trial. J Back Musculoskelet Rehabil. 2020;33. https://doi.org/10.3233/bmr-171077
Müller CEE, Aranha MFM, Gavião MBD. Two-dimensional ultrasound and ultrasound elastography imaging of trigger points in women with myofascial pain syndrome treated by acupuncture and electroacupuncture: A double-blinded randomized controlled pilot study. Ultrason Imaging. 2015;37. https://doi.org/10.1177/0161734614546571
Sancar M, Keniş-Coşkun Ö, Gündüz OH, Kumbhare D. Quantitative Ultrasound Texture Feature Changes with Conservative Treatment of the Trapezius Muscle in Female Patients with Myofascial Pain Syndrome. Am J Phys Med Rehabil. 2021;100. https://doi.org/10.1097/phm.0000000000001697
Ustun B, Yorulmaz E, Geler-Kulcu D. Comparison of Dry Needling and Cold-Spray-Stretching Treatments by Ultrasonography and Electrophysiology: Prospective, Randomized Controlled Trial. J Ultrasound Med. 2024;43. https://doi.org/10.1002/jum.16445
Shomal Zadeh F, Koh RGL, Dilek B, Masani K, Kumbhare D. Identification of Myofascial Trigger Point Using the Combination of Texture Analysis in B-Mode Ultrasound with Machine Learning Classifiers. Sensors (Basel). 2023;23. https://doi.org/10.3390/s23249873 DOI: https://doi.org/10.20944/preprints202311.0618.v1
Kumbhare DA, Ahmed S, Behr MG, Noseworthy MD. Quantitative ultrasound using texture analysis of myofascial pain syndrome in the trapezius. Crit Rev Biomed Eng. 2018;46. https://doi.org/10.1615/critrevbiomedeng.2017024947
Tsai P, Edison J, Wang C, Sefton J, Manning KQ, Gramlich MW. Myofascial trigger point (MTrP) size and elasticity properties can be used to differentiate characteristics of MTrPs in lower back skeletal muscle. Sci Rep. 2024;14. https://doi.org/10.1038/s41598-024-57733-4
Cojocaru MC, Cojocaru IM, Voiculescu VM, Cojan-Carlea NA, Dumitru VL, Berteanu M. Trigger points--ultrasound and thermal findings. J Med Life. 2015;8.
Lewis J, Tehan P. A blinded pilot study investigating the use of diagnostic ultrasound for detecting active myofascial trigger points. Pain. 1999;79. https://doi.org/10.1016/s0304-3959(98)00155-9
Ball A, Perreault T, Fernández-de-las-Peñas C, Agnone M, Spennato J. Trigger Points and Contracture/Contraction Knots: What’s in a Name? Reply to Dommerholt, J.; Gerwin, R.D. Contracture Knots vs. Trigger Points. Comment on “Ball et al. Ultrasound Confirmation of the Multiple Loci Hypothesis of the Myofascial Trigger Point and the Diagnostic Importance of Specificity in the Elicitation of the Local Twitch Response. Diagnostics 2022, 12, 321.” Diagnostics (Basel). 2022;12. http://dx.doi.org/10.3390/diagnostics12102366
Dommerholt J, Gerwin RD. Contracture Knots vs. Trigger Points. Comment on Ball et al. Ultrasound Confirmation of the Multiple Loci Hypothesis of the Myofascial Trigger Point and the Diagnostic Importance of Specificity in the Elicitation of the Local Twitch Response. Diagnostics 2022, 12, 321. Diagnostics (Basel). 2022;12. https://doi.org/10.3390/diagnostics12102365
Dommerholt J. Dry needling - peripheral and central considerations. J Man Manip Ther. 2011;19. https://doi.org/10.1179/106698111x13129729552065
Liu QG, Huang QM, Liu L, Nguyen TT. Structural and functional abnormalities of motor endplates in rat skeletal model of myofascial trigger spots. Neurosci Lett. 2019;711. https://doi.org/10.1016/j.neulet.2019.134417
Margalef R, Sisquella M, Bosque M, Romeu C, Mayoral O, Monterde S, Priego M. Guerra-Perez R, Ortiz N, Tomàs J, Santafe MM. Experimental myofascial trigger point creation in rodents. J Appl Physiol. 2019;126. https://doi.org/10.1152/japplphysiol.00248.2018
Turo D, Otto P, Shah JP, Heimur J, Gebreab T, Armstrong K, Gerber LH, Sikdar S. Ultrasonic tissue characterization of the upper trapezius muscle in patients with myofascial pain syndrome. Annu Int Conf IEEE Eng Med Biol Soc. 2012; 2012. https://doi.org/10.1109/embc.2012.6346938
Shah JP, Danoff J V., Desai MJ, Parikh S, Nakamura LY, Phillips TM, Gerber LH. Biochemicals Associated with Pain and Inflammation are Elevated in Sites Near to and Remote From Active Myofascial Trigger Points. Arch Phys Med Rehabil. 2008;89. https://doi.org/10.1016/j.apmr.2007.10.018
Sikdar S, Ortiz R, Gebreab T, Gerber LH, Shah JP. Understanding the vascular environment of myofascial trigger points using ultrasonic imaging and computational modeling. Annu Int Conf IEEE Eng Med Biol Soc. 2010;2010. https://doi.org/10.1109/iembs.2010.5626326
Bird M, Le D, Shah J, Gerber L, Tandon H, DeStefano S, Srbely J, Kumbhare D, Sikdar S. Characterization of local muscle fiber anisotropy using shear wave elastography in patients with chronic myofascial pain. Ann Phys Rehabil Med. 2018;61. https://doi.org/10.1016/j.rehab.2018.05.029
Hao CJ, Kang XY, Kang CS, Li TT, Huo JZ, Xu Q, Xiao WL, Zhao ZX, Ji XH, Zhang QB. Upper trapezius muscle elasticity in cervical myofascial pain syndrome measured using real-time ultrasound shear-wave elastography. Quant Imaging Med Surg. 2023;13. https://doi.org/10.21037/qims-22-797
Turo D, Otto P, Gebreab T, Armstrong K, Gerber LH, Sikdar S. Shear wave elastography for characterizing muscle tissue in myofascial pain syndrome. Proc Mtgs Acoust. 2013;19. https://doi.org/10.1121/1.4800369
Di Ieva A, Grizzi F, Rognone E, Tse ZTH, Parittotokkaporn T, Rodriguez Y Baena F, Tschabitscher M, Matula C, Trattnig S, Rodriguez Y Baena R. Magnetic resonance elastography: A general overview of its current and future applications in brain imaging. Neurosurg Rev. 2010;33. https://doi.org/10.1007/s10143-010-0249-6
Landgraf MN, Ertl-Wagner B, Koerte IK, Thienel J, Langhagen T, Straube A, von Kries R, Reilich P, Pomschar A, Heinen F. Alterations in the trapezius muscle in young patients with migraine - A pilot case series with MRI. Eur J Paediatr Neurol. 2015;19. https://doi.org/10.1016/j.ejpn.2014.12.021
Sollmann N, Mathonia N, Weidlich D, Bonfert M, Schroeder SA, Badura KA, Renner T, Trepte-Freisleder F, Ganter C, Kieg SM, Zimmer C, Rummeny RJ, Karampinos DC, Baum T, Landgraf MN, Heinen F. Quantitative magnetic resonance imaging of the upper trapezius muscles - assessment of myofascial trigger points in patients with migraine. J Headache Pain. 2019;20. https://doi.org/10.1186/s10194-019-0960-9
Bubnov RV. Evidence-based pain management: is the concept of integrative medicine applicable? EPMA J. 2012;3. https://doi.org/10.1186/1878-5085-3-13
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